|
KMID : 0880220130510060800
|
|
Journal of Microbiology
2013 Volume.51 No. 6 p.800 ~ p.806
|
|
|
A Putative APSES Transcription Factor Is Necessary for Normal Growth and Development of Aspergillus nidulans
|
|
Lee Ji-Yeon
Kim Lee-Han
Kim Ha-Eun
Park Jae-Sin
Han Kap-Hoon
Han Dong-Min
|
|
|
Abstract
|
|
|
|
The nsdD gene encoding a GATA type transcription factor positively controls sexual development in Aspergillus nidulans. According to microarray data, 20 genes that were upregulated by deleting nsdD during various life cycle stages were randomly selected and deleted for functional analysis. None of the mutants showed apparent changes in growth or development compared with those of the wild-type except the AN3154 gene that encodes a putative APSES transcription factor and is an ortholog of Saccharomyces cerevisiae swi4. Deleting AN3154 resulted in retarded growth and development, and the gene was named rgdA (retared growth and development). The rgdA deletion mutant developed a reduced number of conidia even under favorable conditions for asexual development. The retarded growth and development was partially suppressed by the veA1 mutation. The conidial heads of the mutant aborted, showing reduced and irregular shaped phialides. Fruiting body development was delayed compared with that in the wild-type. The mutant did not respond to various nutritional or environmental factors that affected the development patterns. The rgdA gene was expressed at low levels throughout the life cycle and was not significantly affected by several regulators of sexual and asexual development such as nsdD, veA, stuA, or brlA. However, the rgdA gene affected brlA and abaA expression, which function as key regulators of asexual sporulation, suggesting that rgdA functions upstream of those genes.
|
|
|
KEYWORD
|
|
|
Aspergillus nidulans, APSES transcription factor, rgdA, asexual development, VeA
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|